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Shaking his head Harry pulled out the Potion's book from his pocket, nodded at the Baron and Hermione and opened it to where he had left off. Clearing her throat McGonagall threw glares across the Great Hall to get them to shut up so she could finish the Sorting, continuing down the list there were no more great surprises; Harry and Hermione ...

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Overview of the screening strategy for the study of essential genes. a Homozygous phb2(tm2998);Phsp6::GFP mutants are sorted at L2 stage from a mixed population of balanced heterozygous phb2(tm2998)/mIn1 animals into multiwell plates using the COPAS Biosort "worm sorting".b Next, bacteria containing the OrthoList RNAi sublibrary are added to the wells and worms .

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Therefore, a total of 12 zebrafish genes were selected for functional screening (Table III in the onlineonly Data Supplement). To test whether the candidate genes are expressed in the zebrafish endothelium, we isolated ECs from 26 hpf flk1:EGFPNLS embryos using fluorescenceactivated cell sorting (Figure VII in the onlineonly Data Supplement).

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